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Registros recuperados: 190 | |
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Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio. |
Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced... |
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Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus. |
Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532 |
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OKAWARA, Sumika; HAMANO, Seizo; TETSUKA, Masafumi; 手塚, 雅文. |
Glycerol plays multi-functional roles in cellular physiology. Other than forming the backbone molecule for glycerophospholipid and triglyceride (TG), glycerol acts as an energy substrate for glycolysis. Spermatozoa are known to utilize glycerol for energy production, but there are no reports of this in oocytes. In this study, the value of glycerol as an energy substrate for bovine oocyte maturation (Exp. 1) and the gene expression of glycerol kinase (GK), an enzyme crucial for cellular glycerol utilization, in bovine oocytes and early embryos (Exp. 2) were examined. In Exp. 1, in vitro maturation (IVM) was conducted using synthetic oviduct fluid supplemented with/without glucose (1.5 mM) and/or glycerol (1.0 mM), and maturation rate, degree of cumulus... |
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Palavras-chave: Bovine; Embryo; Glycerol; Glycerol kinase (GK); Oocyte maturation. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2829 |
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Martins,E.; Martins,V.M.V.; Marques Júnior,A.P.; Vasconcelos,A.C.; Chiarini-Garcia,H.; Malard,P.F.. |
This work evaluates the preservation of bovine placentomes using paraformaldehyde and glutaraldehyde at 4% and solutions of Karnovsky, Bouin and Carnoy for 4, 12 and 24 hours fixation, at room temperature or at 4ºC, before inclusion in plastic resin and paraffin. The best preservation of the specimens was obtained after 12 hours of fixation with those solutions containing aldehydes. With Bouin solution the best time of fixation was 4 hours, while for Carnoy solution the best time of fixation was 24 hours. Inclusion in plastic resin gave better results than in paraffin, and the temperature did not influence the quality of preservation of the specimens of bovine placentomes for evaluation with light microscopy. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Placentome; Light microscopy. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000200006 |
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EL-KHODERY, Sabry; YAMADA, Kazutaka; AOKI, Daisuke; KAMIO, Kyouhei; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; ISHII, Mitsuo; INOKUMA, Hisashi; YAMAUCHI, Sen-ichi; MATSUI, Takane; 山田, 一孝; 古林, 与志安; 石井, 三都夫; 猪熊, 壽. |
Computed tomography (CT) was used for diagnosis of brain abscess in a 6-month-old, Japanese black calf presented with neurological dysfunction, compulsive circling and vision disturbance. CT images showed asymmetric lateral ventricles, and presence of intra-cranial multiple low absorption lesions surrounded by capsule suggestive of abscess in the right cerebral hemisphere. Postmortem examination revealed marked swelling of right cerebral hemisphere and olfactory bulb. Multilocular large abscess containing creamy pus was found to occupy most area of periventricular and lateral ventricle. Fusobacterium necrophrum was isolated from the abscess contents as the causative agent. These results demonstrate that CT is useful tool for tentative diagnosis of bovine... |
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Palavras-chave: Bovine; Brain abscess; CT. |
Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3926 |
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Martinez Castro, Cesar Julio; Cotera Rivera, Julian; Abad Zavaleta, Jose. |
The objective of the present study was to make a diagnostic and characterization of the production and marketing of bovine milk in double purpose production systems, also known as tropical dairy, in the premises of Dobladero in José Azueta Veracruz, Mexico. Thirty Milk producers from the Local Livestock Association (Asociación Ganadera Local) were interviewed from May to July 2010. The results show that the production units are common parcels, with traditional pasturage on rain-fed conditions in a dry tropical weather, and supplementary feeding during dry seasons. A mixture of Zebu and Swiss cattle breeds predominate along with other europeans; the cow milking takes place daily in the morning and manually by the same producer and with the help of their... |
Tipo: Article |
Palavras-chave: Bovine; Milk; Production; Marketing; Double purpose cattle raising.; Agribusiness. |
Ano: 2012 |
URL: http://purl.umn.edu/120307 |
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Oliveira, Wagner Dias Coelho de; Dias e Silva, Tairon Pannunzio; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Oliveira, Ronaldo Lopes; Bezerra, Leílson Rocha. |
This study was conducted to evaluate changes in hematological biomarkers of Nellore cows at different reproductive stages. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following hematological biomarkers: hemogram (red blood cells, globular volume, hemoglobin, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC)) and leukogram (total and differential counts). The erythrogram was influenced (p < 0.001) by the reproductive stages, with variation in the results obtained for the total... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood; Bovine; Erythrogram; Leukogram; Postpartum; Prepartum.. |
Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45725 |
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Yoda, Tomoko; Sakurai, Haruhisa; Igarashi, Ikuo; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 依田, 知子; 櫻井, 治久; 五十嵐, 郁男; 小俣, 吉孝; 齊藤, 篤志; 鈴木, 直義. |
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Palavras-chave: Toxoplasma; Serum components; Bovine; Toxoplasma lysate antigen; Lymphokines. |
Ano: 1990 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1569 |
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SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫. |
The aim of the present study was to examine the messenger RNA expressions of the endothelin and angiotensin systems during the periovulatory phase in gonadotrophin releasing hormone (GnRH)-treated cows. Ovaries were collected by transvaginal ovariectomy (n=5 cows/group), and the follicles (n=5, one follicle/cow) were classified into the following groups: before GnRH administration (control, before LH surge), 3-5 h after GnRH (during LH surge), 10 h after GnRH; 20 h after GnRH, 25 h after GnRH (peri-ovulation), and early corpus luteum (CL) (Days 2-3). Expression of mRNA was investigated using quantitative real-time PCR. The expression of angiotensin converting enzyme (ACE) mRNA significantly decreased immediately after onset of the LH surge and remained at... |
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Palavras-chave: Angiotensin; Bovine; Endothelin-1; Gonadotrophin releasing hormone (GnRH); Ovulation. |
Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2987 |
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Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia. |
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015 |
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Maeda,Emilyn Midori; Zeoula,Lucia Maria; Jobim,Clóves Cabreira; Bertaglia,Franciele; Jonker,Roberto Cornelis; Geron,Luiz Juliano Valério; Henrique,Douglas Sampaio. |
The objective of this study was to evaluate the sugarcane silage in relation to chemical composition, pH, total losses, aerobic stability, in vitro digestibility of dry matter (IVDDM) and effective degradability (ED) of bovine and buffaloes under the following treatments (on a dry matter (DM) basis): sugarcane silage control (control), with addition of 3.3% urea; with addition of Lactobacillus buchneri (inoculate); with inoculate + 3.3% urea; with inoculate + 25.0% soybean hull, and with inoculate + 25.0% cassava byproduct meal. The experiment was analyzed through a completely randomized design with three replications, and for DM ED, a double 4 × 4 Latin square with a 4 × 2 factorial arrangement was used. The sugarcane variety was the RB-855156... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Buffaloes; Cassava byproduct meal; Lactobacillus buchneri; Soybean hulls. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001200034 |
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Falavigna-Guilherme,A.L.; Silva,K.; Araújo,S. M.; Tobias,M.L.; Falavigna,D.L.M.. |
The aim of this work was to verify the occurrence of bovine and swine cysticercosis in animals slaughtered at the Abatedouro Municipal de Sabáudia, Paraná State. The number of daily-abated animals, the number of animals with cysticercus and the number and location of the cysticercus in the animals in a period of twelve months were collected. From 1046 swines slaughtered none showed cysticercus, but 36/out of 389 bovines (9.3%) were contaminated. Head was the most parasited region, followed by heart, tongue and liver. The absence of contamination observed in swines and the low contamination of the bovines reinforce the efficiency of the control measures accomplished in Paraná State. |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Swine; Bovine; Cysticercosis; Slaughterhouse; Paraná State. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500036 |
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NISHIMOTO, Hiromi; HAMANO, Seizo; HILL, Glen Alan; MIYAMOTO, Akio; TETSUKA, Masafumi; 宮本, 明夫; 手塚, 雅文. |
A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified... |
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Palavras-chave: Bovine; Follicular fluid; Follicle classification; Glucose; Lactate; Steroid. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2828 |
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Registros recuperados: 190 | |
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